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首頁 > 技術(shù)文章 > BioLife Solutions CS10 210102凍存液

BioLife Solutions CS10 210102凍存液

更新時間:2017-11-23瀏覽:4557次

BioLife Solutions CryoStor CS10 細胞凍存液預混10% DMSO,適用于凍存多種類型細胞。

 

 

品牌:BioLife Solutions

貨號:PART No. 210102

容積:VOL. 100mL

型號:CryoStor CS10

 

 

BioLife Solutions CryoStor CS10細胞DMSO凍存液210102訂購信息

 

品牌

貨號

產(chǎn)品描述

規(guī)格

BioLife Solutions

210102

CryoStor CS10 Freeze Media 細胞DMSO凍存液

100mL /瓶

 

英文名BioLife Solutions CryoStor CS10 Freeze Media

中文名:BioLife Solutions CryoStor CS10 細胞DMSO凍存液

 

BioLIfe Solutions CryoStor CS10細胞DMSO凍存液產(chǎn)品系列

 

 

各種規(guī)格的BioLIfe Solutions CryoStor 細胞DMSO凍存液

 

品牌

貨號

產(chǎn)品描述

規(guī)格

BioLife Solutions

210102

CryoStor CS10 Freeze Media 細胞DMSO凍存液

100mL /瓶

BioLife Solutions

210210

CryoStor CS10 Freeze Media 細胞DMSO凍存液

1000mL/袋

BioLife Solutions

210202

CryoStor CS10 Freeze Media 細胞DMSO凍存液

100mL/袋

BioLife Solutions

210374

CryoStor CS10 Freeze Media 細胞DMSO凍存液

16mL /瓶

BioLife Solutions

210373

CryoStor CS10 Freeze Media 細胞DMSO凍存液

10mL /瓶

 

BioLife Solutions CryoStor CS10細胞DMSO凍存液210102 代理銷售歡迎您致電 華雅再生醫(yī)學旗艦公司:紅榮微再(上海)生物工程技術(shù)有限公司  :152 1681 4001。紅榮微再-客服: 316 808 6348  經(jīng)銷商專員

 

BioLife Solutions CryoStor CS10 Freeze Media 使用與凍存方法

CryoStor® Usage and Cryopreservation Protocol

細胞準備

1) Place cells to be cryopreserved into suspension (mechanical or enzymatic dissociation)

2) Centrifuge cells to obtain cell pellet

3) Remove supernatant - Note: Remove as much culture media as possible, to reduce dilution of CryoStor® solution.

加CryoStor凍存液

4) ISOLATION: Add cold (2-8°C) CryoStor®

a. Cell concentrations: 0.5-10 x 106

 cells/ml for routine cell culture protocols (higher [cell] possible).

b. DMSO is pre-mixed in CryoStor® - no additives are necessary.

5) PRE-FREEZE: Incubate cell suspension at 2-8°C for approximay 10 minutes

6) NUCLEATION: Freeze samples at -70°C (many protocols utlilize -70°C and -80°C interchangeably)

a. Use a controlled rate freeze (-1°C/min) or similar protocol for most mammalian cell systems.

b. The freezing device or isopropanol container should be pre-cooled to 2-8°C.

c. Ice nucleation within the sample (seeding) should be initiated at approximay -5°C using either a liquid nitrogen burst program setting on a controlled rate freezer or mechanical agitation (flick or tap) of the cryovial/sample container after approximay15-20 min. at -70°C.

d. Freeze time (-70°C) using isopropanol containers is recommended to be 3-4 hours.

BioLife Solutions 儲存

7) STORAGE: Place samples into storage

a. Store samples at liquid nitrogen temperatures (below -130°C).

b. Sample storage at -80°C is only recommended for short-term storage (weeks to months).

細胞復蘇解凍

8) THAWING: Thaw samples quickly in a 37°C water bath

a. Sample thawing should be conducted with gentle swirling of sample until all visible ice has melted. Approximate thaw time for a 1 ml sample in a cryovial is approximay 3 minutes.

b. DO NOT allow sample to warm above chilled temperatures (0-10°C). Cryovials should be cool to the touch when removed from bath.

Passive thaw is not recommended.

9) Dilute cell/CryoStor® mixture immediay with culture media

a. Dilution procedure can be preformed in a single step.

b. The dilution media should be between 20°C and 37°C.

c. A dilution ratio of 1:10 (sample to media) or greater is recommended.

10) Plate cells in appropriate configuration

11) Place cells into culture conditions or utilize immediay

12) Viability assessment 24-hours post-thaw*

BioLife Solutions Note: To obtain an accurate measure of cell viability following cryopreservation, assessment should be performed 24 hours post-thaw and compared to non-frozen controls.

*Sample assessment immediay post-thaw with membrane integrity indicators, such as Trypan Blue, for comparative analysis of sample cell yield and viability often results in significant overestimates of cell survival.

Live/Dead fluorescent assays or metabolic assays (MTT or alamarBlue®) are recommended for more accurate viability assessment.

Visual inspection of adherent cells and cells “floating” in the media is also recommended.

 

BioLife Solutions CryoStor CS10細胞DMSO凍存液210102 代理銷售歡迎您致電 華雅再生醫(yī)學旗艦公司:紅榮微再(上海)生物工程技術(shù)有限公司  :152 1681 4001。紅榮微再-客服: 316 808 6348  經(jīng)銷商專員

 

 

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