亚洲欧洲精品一区二区三区不卡_人人澡人人妻人人爽_久久久久久久久91_少妇久久久久久久久久

歡迎光臨紅榮微再(上海)生物工程技術(shù)有限公司!
服務熱線15021010459
Article技術(shù)文章
首頁 > 技術(shù)文章 > 人AB血清用于人體外終末期紅系分化的論文

人AB血清用于人體外終末期紅系分化的論文

更新時間:2017-10-25瀏覽:1727次

AB血清對人體外終末期紅系分化進程的影響

摘要

 

目的:探究體外誘導造血干細胞向紅系分化過程中人AB血清對終末期紅系分化進程的影響。

 

方法:從臍帶血中用免疫磁珠法分離提取CD34+細胞,體外誘導其向紅系分化。根據(jù)培養(yǎng)體系中是否添加人AB血清,設(shè)置實驗組為添加人AB血清組,對照組為不添加人AB血清組。通過檢測細胞表面紅系分化標志分子糖化血紅蛋白A(glycophorin A,GPA)、人紅細胞陰離子交換蛋白Band3及整合素α4(α4-integrin)的表達反映終末紅系分化進程,用流式細胞術(shù)檢測紅系細胞終末分化脫核情況。結(jié)果:與對照組相比,實驗組GPA陽性細胞百分率較高,Band3表達較高,而α4-integrin表達較低(P<0.05)。實驗組細胞脫核百分率明顯高于對照組(P<0.05)。結(jié)論:在體外紅系發(fā)育培養(yǎng)體系中,人AB血清對終末期紅系分化進程有一定的促分化、促脫核作用。

 

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2016 Dec 28;41(12):1245-1251. doi: 10.11817/j.issn.1672-7347.2016.12.001.

[Effect of AB serum on human terminal erythroid differentiation ex vivo].

[Article in Chinese; Abstract available in Chinese from the publisher]

Liao J1,et al.

Abstract in English, Chinese

OBJECTIVE:

To demonstrate the effect of AB serum on terminal erythroid differentiation ex vivo.? Methods: After separation of CD34+ cells from cord blood, the cells were cultured and divided into a control group and an experimental group. The effects of AB serum were examined by the expressions of different markers (GPA, Band3 and α4-integrin) for erythroblast differentiation and enucleation by flow cytometry. Results: The CD34+ cells were successfully differentiated to enucleated red blood cells. There were evident differences among the expressions of GPA, Band3 and α4-integrin between the 2 groups. The percentage of GPA positive cells in the experimental group was bigger than that in the control group in every time point. The expression of Band3 in the experimental group was higher than that in the control group. The expression of α4-integrin in the experimental group was lower than that in the control group. In addition, the enucleation rate in the experimental group was higher than that in the control group.? Conclusion: AB serum can promote the cell differentiation and enucleation during terminal erythroid differentiation in vitro.

Free full text

原版中文

>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>

歡迎您致電咨詢Human Serum AB人AB血清 華雅再生醫(yī)學旗艦公司:紅榮微再(上海)生物工程技術(shù)有限公司 :152 1681 4001。華雅再生醫(yī)學-客服: 316 808 6348

 

GemCell™人AB血清來自于美國FDA認證的AB血型健康男性供體。供體AB血漿加抗凝劑經(jīng)去纖維蛋白處理得到AB血清,一致性較高。所有供體的病毒標志物檢測都是陰性。

人AB血清作為牛血清(FBS、FCS)的替代物,不含外源物,適合組織工程、移植和細胞治療領(lǐng)域的間充質(zhì)干細胞擴增,及其他免疫系統(tǒng)相關(guān)的細胞培養(yǎng)和反應。

 

產(chǎn)品訂購信息

品牌

貨號

產(chǎn)品描述

包裝

GemCell

100-512

U.S. Origin Human Serum AB人AB血清

100 mL/瓶

 

紅榮微再(上海)生物工程技術(shù)有限公司以“傳遞科學價值,服務科學研究”為宗旨,主營:干細胞、醫(yī)療、細胞治療、器官再生四大板塊的產(chǎn)品。

 

91精品国产高清91久久久久久| 国精产品一区二区三区有限公司 | 欧美三级在线| 啊灬啊灬啊灬深高潮了| 玩弄少妇人妻500系列视频| 黑人狂躁女人高潮视频| 久久国产一区二区| 国产淫妇xxxxxx丰满老熟妇 | 国产乱码精品一区二区三区五月婷| 国产激情在线播放| 国产又粗又猛又爽又黄的| 亚洲一区二区黄色| 国产精品一区二区三区免费观看 | 成人精品一区二区三区| 激情五月婷婷丁香| 国产老熟女伦老熟妇露脸在线| 人妻激情偷乱一区二区三区| 人妻视频一区二区| 国产精品99久久久久久武松影视| 伊人色综合久久| 国产精品久久一区| 又色又爽又黄18网站| 亚洲精品一区二区三区三| 中文字幕一区二区三区精华液| 日韩一区二区中文字幕| 久久久久久免费毛片精品| 日本一区二区不卡视频| 中文字幕在线乱码人妻app | 少妇高潮久久久久久毛片| 91人妻人人澡人人爽人人麻豆| 国产无遮挡又黄又爽在线观看| 亚洲欧美日韩精品永久在线| 亚洲视频在线一区| 黄色激情五月天| 成人福利在线观看| 赘婿动漫在线观看免费完整版| 国产精品一区久久久久| 欧美熟妇精品黑人巨大一二三区| 亚洲蜜臀av乱码久久精品蜜桃| 狠狠人妻久久久久久综合果冻| 免费av日韩|